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Features

• Cost effective alternative to ELISA
• Single-plate protocols for HTS
• Highly sensitive: < 39 pg/mL
• Small sample size needed: down to 5 μL
• Compatible with cell supernatant and whole cells

Applications

• Human IL5 quantification on cell supernatants and whole cells
• Functional screening of compounds using IL5 secreting cells

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Human IL5 is measured using sandwich immunoassay involving two monoclonal antibodies, one labelled with Eu-Cryptate (Donor) and the second with d2 (Acceptor). The intensity of the signal is proportional to the concentration of the IL5 present in the sample.

A Human IL5 test, like all cytokine assays, can be run with cell supernatants or with whole cells using the protocol described on the left. The antibodies labelled with HTRF? fluorophores may be pre-mixed and added in a single dispensing step to further streamline the protocol. An assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally. The Human IL5 assay protocol is described here, using a white 384-well small volume plate.

As described on the figure on the right, the performances of the assay are not affected by culture media (RPMI + 10% FBS)

Description                                        Cat. No

Human IL5 kit - 1,000 tests          63ADKPEBU007

Human IL5 kit - 20,000 tests         63ADKPECU007

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